For all your measurements , start the reaction by adding ADH and follow the change in absorbance , note time [min] . Keep the enzyme and NAD on ice until used . The concentration of ethanol - the substrate - in the series of reaction mixtures is 0 .5 mM , 1 mM , 2 .5 mM , 5 mM , 10 mM and 20 mM . To start the reaction - add the ADH to the cuvette , mix the cuvette contents and replace in spectrophotometer . Record the data for a period...
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