Most Probable Number (MPN ) Method In executing the MPN method , a water sample is diluted ten-fold in a series and inoculated in 1-ml aliquots into a medium . After a period of incubation (approximately 24 hours , the samples are examined for colony growth . Growth is theoretically probable if one organism is present in each sample . The greater the number of samples done from each dilution , the better the statistical accuracy becomes . When the incubation period ends , patterns are noted regarding growth...











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