P2X2 receptor subunit immunoreactivity in the NTS and DVN when we used diaminobenzidine (DAB ) or fluorescent secondary antibody , and the same result was observed in tissue sections obtained from 12 animals in 12 separate experiments . The specificity of the P2X2R antibody was compared between two different P2X2 R antisera ( neuromics P2X2 R and Alomone . The same pattern of staining was observed with either antisera , although the intensity of the staining was almost higher with neuromics one in all examined areas...











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