microbiology/nursing

Running head : HIV-1 CLONING VECTORS

Article Review : HIV-1 Cloning Vectors for Antiretroviral Resistance Testing

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Article Review : HIV-1 Cloning Vectors for Antiretroviral Resistance Testing

In a journal article by Sutton and associates , a better technique for antiretroviral resistance testing was proposed . The researchers tried to propose the said methodology due to the difficulty and slowness of the present techniques in resistance testing of Human Immunodeficiency Virus Type 1 or HIV-1 (Sutton , 1999

The antiretroviral resistance testing of HIV-1

involves the cloning of HIV-1 PCR products from the patients ' specimens . After which , from pooled populations , genotyping and phenotyping are done by sequencing in to detect mutations of the virus . These processes are usually carried out by standard ligase-mediated cloning which is a very slow and complicated process . Furthermore , there are times that minority detections are not identified by this method (Sutton , 1999 . It is for this very reason that the researchers constructed a more efficient and useful method . The method employs a simple HIV-1 sequence specific uracil deglycosylase-mediated cloning method by means of vectors and primers (Sutton , 1999 . Although there is no explicitly stated hypothesis , the objective of the study is to prove that the proposed technique is more advantageous than that of the standard technique used presently . Results of the study favorably exhibit that the researchers method is a better way of cloning the virus as well as a better way of detecting resistant mutations . The new technique is more rapid...