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WRITING CRITIQUES OF SCIENTIFIC LITERATURE

Article Critique

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26 January 2009

Kofron , M , Wylie , C Heasman , J (2004 . The Role of Mixer in Patterning the Early Xenopus Embryo . Development 131 , 2431-2441

The purpose of the article is to establish the role that is played by Mixer in the boundary formation of mesoderm and endoderm in the Xenopus The Mixer transcription factor is expressed in proteins and VegT . Thus does the blocking of the translation of Mixer protein during expression

br at the time of gastrulation have any effect ? The authors found that the embryos that were depleted by Mixer had abnormalities . Thus , the effect of the Mixer-depletion influences the gene rather than the germ layer Hence , the authors ' main objective is to determine the major role that is played by Mixer in controlling the degree in which the cells induce formation of the mesoderm . Thus , were the authors ' successful

The sample eggs were obtained from injecting Xenopus laevis and were then fertilized . The embryos were injected with Mixer Mo . The vegetal and animal pieces that were obtained were either kept differently or combined and then they were cultured . The vegetal cells were removed from the mixture while the animal caps were cultured . The vegetal cells were then transplanted to the host blastulae and embryos were embedded in wax and dissected . Three overlapping layers of morpholino oligos were developed . The oligo was then injected to four animal blastomeres Biotin in vitro Translation kit (Roche...

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