Purification of Alcohol dehydrogenase from baker's yeast

Purification of Alcohol Dehydrogenase from Baker 's Yeast

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Purification of Alcohol Dehydrogenase from Baker 's Yeast

Introduction

Proteins are complex organic molecules that are found in many living and non-living systems . Most systems , especially living , are made up of complex macro-molecules , among which proteins are well prominent Usually , these systems contain different kinds of proteins , among which the scientist is interested in one or a few of the different molecules present in the specimen . The process of separation of

purification of proteins is aimed at bringing out a particular type of protein out of a complex mixture . This process would aid in the study and analysis of the structure and function of the protein molecule . Usually , the specimen which is started with is a tissue sample or the culture of a particular microorganism

To separate proteins , one or more tests , referred to as assays , would need to be carried out . There are numerous assay methods ranging from salting out methods to chromatographic techniques . However , the kind of procedure that would be used for the separation and purification process would be determined by the nature and characteristics of the protein of interest . In separating and purifying proteins , assays can be conducted on the enzymatic activity of the protein and its concentration during the separation or purification process

Most proteins are usually bound together with other proteins to form large macromolecules . Usually , they may be found free in a particular solution or embedded deep...