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Paper Topic:

Plant Pigementation


Plant Pigment Chromatography


To use the process of chromatography to separate plant pigments

To compare the plant pigments found in spinach and red leaf lettuce leaves

Alternate Hypothesis

The major plant pigment extracted from leaf lettuce will travel faster in chromatography compared to the spinach extract

Plant pigments found in red lettuce and spinach will differ in quantity .Discussion Questions

Describe what each of your chromatography strips looked like Specifically , identify the pigments on each strip and compare their positions to one another

Plants have

four types of pigments , namely chlorophyll , carotenoids anthocyanins , and xanthophylls . These pigments have different polarities and chemical properties . In chromatography , the pigments will separate based on their affinity to the medium , and affinity for the solvent . The solvents used in this experiment are water (polar ) and acetone (mid-polar . Therefore , the different pigments will migrate based on their respective polarities too . The pattern of migration will be similar for acetone and distilled water . The fastest to migrate (or found at the topmost of the strip ) will be anthocyanin , followed by carotenoids , then xanthophylls and lastly chlorophyll . Chlorophyll is insoluble in polar solvents therefore it will migrate slowest in both water and acetone and will be found closest to the bottom of the strip

Which pigments did the spinach and the red leaf lettuce have in common Propose an explanation for this Spinach and red lettuce will have the same pigments , only they will be in different concentrations . Plants have...

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