Pharmacology LABORATORY RESEARCH REPORT

REPORT

Experiment I Partition Coefficient

Results Absorbance after shaking

Measured ph volumes sulphadimidine acetyle sulphadimidine

9 .67 0 .67 1 .24

8 .40 1 .00 1 .60

7 .31 0 .260 0 .63

6 .11 0 .32 0 .36

4 .91 0 .33 0 .38

3 .20 0 .33 0 .37

2 .80 0 .30 0 .38

1 .95 0 .33 0 .49Graph for Experiment I Absorbance of original octanol solutions of the drugs .023

Suphadimidine

1 .385

Acetyl sulphadimidine 1 .56

? at 267mm for sulphadimidine

? at 266 for acetylsulphamidine

- clear

K value -40

Comment : Partition coefficient for sulphadimine and acetyl sulphadimine , the two drugs chosen has been found to be similar at lower volumes with very close or similar absorbance after shaking values However at higher ph volumes absorbance after shaking seems to vary considerably for the two drugs and is clearly seen in the graph in which for the higher ph volumes the graphical presentation of the two drugs show variations whereas for the lower ph volumes the absorbance after shaking values of the two drugs are nearly similar

Experiment II

Run A

Time absorbance Loge In (of absorbance

2 0 .26 -1 .35

4 0 .21 -1 .56

6 0 .21 -1 .56

8 0 .161 -1 .83

10 0 .11 -2 .21

12 0 .1 -2 .30

14 0 .08 -2 .53

16 0 .06 -2 .81

18 0 .05 -3 .00

20 0 .05 -3 .00

22 0 .04 -3 .22

24 0 .04 -3 .22

26 0 .04 -3 .22

28 0 .03 -3 .51

30 0 .03 -3 .51 Slop -0 .08133 Run B

Run B time absorbance Loge In (of absorbance

2 0 .39 -0 .94

4 0 .54 -0 .62

6 0 .38 -0 .97

8 0 .37 -0 .99

10 0 .38 -0 .97

12 0 .37 -0 .99

14 0 .36 -1 .02

16 0 .38 -0 .97

18 0 .4 -0 .92

20 0 .4 -0 .92

22 0 .5 -0 .69

24 0 .49 -0 .71

26 0 .5 -0 .69

28 0 .4 -0 .92

30 0 .5 -0 .69 Slop 0 .0063 s-1 Run C

time absorbance IN absorbance

2 0 .26 -1 .35

4 0 .31 -1 .17

6 0 .21 -1 .56

8 0 .16 -1 .83

10 0 .21 -1 .56

12 0 .18 -1 .71

14 0 .22 -1 .51

16 0 .17 -1 .77

18 0 .17 -1 .77

20 0 .08 -2 .53

22 0 .09 -2 .41

24 0 .06 -2 .81

26 0 .06 -2 .81

28 0 .07 -2 .66

30 0 .08 -2 .53 slop -0 .05676 s-1 Comments

Run A shows log in absorbance decreasing in value with time as with an increase of time from 2 to 4 and up to 30 mins , the absorbance value decreased drastically from .26 to .03 . The Log in Absorbance value also decreased significantly from -1 .35 to -3 .5 showing a drop towards negative values in absorbance with time . The graph has been plotted accordingly

Run B shows a stable and not too drastically decreasing absorbance values with time as there has been increase of absorbance values in certain cases showing a rather uneven graph with occasional increase and decrease of values in absorbance with change of time from 2 mins to 30 In fact the log in absorbance rates varied from -0 .92 to -0 .69 varying between a low of -1 .02 and a high of -0 .62 . The graph as plotted here shows uneven instead of a straight line in contrast to Run A

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