Liquid Chromatography

The instrumental method of determining the Scoville Heat Value (SHV ) of various capsanoid substances have several advantages . The first one is the sample can be tested immediately without any pre-treatment . Another advantage is that the results can be obtained and analyzed in about four hours , which is a relatively short amount of time . Finally , the instruments used for the experiment has an adequate sensitivity for determining the SHV capsanoid in common food products

However , this method is not without its limitations . Background noises may appear in the graphical result near the 280-nm

wavelength which can be hard to be distinguished from the capsanoid peaks if the sample is not strong enough . Pressure is also a limitation of this method if particle based columns are used . Using a column packed with larger diameter particles reduce the limitation of column length and flow rates but cannot handle large pressures . Because the mobile phase has to travel through larger particles , the flow rate can be slowed down to allow better separation on the retention time . On the other hand , using smaller particles columns would limit column length and flow rates despite the ability to handle higher pressures . The adverse effect is that it could be harder to separate the retention time of the mobile phase because of the low column length and high flow rate

The effect of the composition of the mobile phase on the retention time is what makes liquid chromatography works . The retention time of a solution is unique to...