Gas Chromatography

Name

Professor

Subject

Date

Potential Application of Gas Chromatography /Tandem Mass Spectrometry

in the Measurement of Coeluting Isomers

Introduction

Chromatography is the most commonly used separation method in the diverse field of sciences . Any chromatographic technique yields to an efficient separation of mixture into its components . However chromatographic resolution is often affected by inseparable constituents under chromatographic conditions . This limitation can be mended by employing Mass Spectroscopy . On the other hand , mass spectroscopy has the capability to differentiate non-isomeric coeluting substances based on their pros of

fragmented ion . Thus , the analysis of chromatographically inseparable constituents is often coupled with mass spectroscopy . Yet , the conventional mass spectroscopy can hardly contrast the isomeric fragmented ions , hence , Zeng , Chou , and Yu (2002 aimed to design analytical scheme that can possibly identify two coeluting isomers through gas chromatography-mass spectroscopy , GC-MS in just one chromatographic column

Objectives

In spite of the collaborative efforts catered for the design of a method specific for polychlorinated biphenyl or PCB congener , the development of a single separation column for the known 209 PCB congeners in a single run approach was a failure . Meanwhile , the multidimensional gas chromatography employed in PCB congeners ' coelution is not feasible in most analytical laboratories due to complexity of techniques . Also , the large variation of every PCB congener in terms of biodegradation and toxicity implies the needs for concentration detection specific to every PCB congener in environmental samples . Hence , this study is an applied research for after the development of method specific to PCB congeners...