Commd1 Gene

COMMD1 GENE (Name of author (Name of institution

COMMD1 location

COMMD 1 gene lies on chromosome 2 .Its location is 2p15

The designation of homologs of MURR1 identified here required the generation of a new nomenclature . Murr1 derived its name from its proximity to the U2af1-rs1 locus in mice (mouse U2af1-rs1 region 1 however , this genomic organization is not observed in other organisms including humans . In addition , an unrelated gene that also lies in close proximity to U2af1-rs1 has been designated Murr2 , precluding the use of this name for MURR1 homologs

.In consultation with the HUGO gene nomenclature committee , the term COMMD (COMM Domain containing ) is proposed to designate these factors based on the shared structural domain that defines this family of proteins and has been adopted in NCBI public databases . The name COMMD1 is suggested for MURR1 as a means to standardize the nomenclature to designate this protein family and will be used hereafter

Genomic location : bands according to Ensembl , locations according to Geneloc Gene Loc location for GC02P062044 (about GC identifiers

Start : 61 ,986 ,292 bp from pter

End : 62 ,216 ,709 bp from pter

Size : 230 ,417 bases

Exon structure

From Entrez Gene

Id Chromosome Contig Strand Exon Start Exon End

150684 2

150684 2

150684 2

150684 2

150684 2 From HYPERLINK "http /www .ensembl .org /Homo_sapiens /geneview ?gene ENSG Ensemble

Id Chromosome Contig Strand Exon Start Exon End

ENSG 2

ENSG 2

ENSG 2 The structure N-commd1 has a ? helical fold with little resemblance to other ? helical proteins .The compact nature of N-COMMD1 suggests that full length proteins are modular with a precise function for each domain . Several areas are positively charged which provide an area for interaction with other negatively charged areas in COMMD proteins (Sommerhalter . Zhang . Rosenweig .2007

COMMD1 RNA is expressed in the liver as well as many other tissues COMMD1 contains no copper binding motifs but it does contain the COMM domain . The COMM domain is leucine rich and has approximately 85 amino acids (Klomp , Van De Sluis . Wijmenga . Di Bie , 2005 ) this domain is present in 9 other human genes COMMD 2-10 . All Commd proteins have been found to complex with COMMD 1 although the exact structure of these complexes in vivo needs to be determined . The exact molecular function of COMMD1 remains unknown however it is seen in a number of cellular processes

COMMD-COMMD interactions . Each of the 10 COMMD proteins was expressed in 293 cells in fusion with GST and precipitated (IP ) from cell lysates by glutathione-Sepharose beads . The presence of endogenous COMMD1 in the precipitates was determined by immunoblotting . B , schematic representation of COMMD1 and the amino acid residues that are the boundaries for interaction mapping experiments . C and D , the COMM domain is required for the association of COMMD proteins . COMMD1-Flag (C ) or COMMD3-FLAG (D ) were expressed in 293 cells along with various regions of COMMD1 in fusion with GST as indicated . COMMD1-GST was precipitated from cell lysates by glutathione-Sepharose beads and the presence of COMMD1...