Paper Topic:

Calculating concentration using spectrophotometry.

Subject : chemistry

Lecturer : XYZ

Calculating concentration using spectrophotometry

Student name

ID : 123456

Lab class

DATE

Lab report

Introduction

The discovery of spectrophotometer has made it possible for the quantification of material using electromagnetic spectra which is measured as absorbance /transmittance . Photometry principle is applied by measuring the amount of light as a function of the light wavelength Spectrophotometer operate by measuring amount of light that is able to pass through which is reflected on the detector , to accomplish the task a suitable wave length is

selected which can be detected by the human eye on the measuring device normally between 780-380nm Spectrophotometer has been used to calculate concentration of substance by using their absorbance (Daniel 2003

The application of the Beer-Lambert law which define a logarithmic relationship between the light transmissions through a substance and absorption coefficient , distance travelled by the light source or the diameter of the cuvette and the concentration of the substance , for a colored solution the amount of light intensity is reduced because of absorption . The Beer-Lambert equation is given by A ? c where A is the absorbance ? is the absorption coefficient ? is the distance travelled by light and c is the concentration of the solute (Clark 2007 .This task is accomplished by plotting a standard curve and calculating the concentration using Beer-Lambert law which is used to identify of the unknown

The aim of the experiment was to understand the principles of spectrometry and to identify the effectiveness of either of the two spectrometric procedures...

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